ORGANIZATIONAL DEVELOPMENT Question # 1 Essay Example | Topics and Well Written Essays - 250 words

ORGANIZATIONAL DEVELOPMENT Question # 1 - Essay Example As such, it is important for the management to come up with strategies to change job satisfaction into positive employee behavior. To begin with, the manager must come up with means of rewarding employees who register exemplary performance. This will increase the employee satisfaction levels of such employees as they feel that their hard work is being appreciated by the organization. The manager should also involve employees in decision making especially on matters that directly affect them (Robbins & Judge, 2011). This will make employees feel like being part of the organization thus boosting their morale. Further, managers need to come up with occasional surveys to determine employee satisfaction levels. This will enable the management ascertain areas, which they need to improve in order to satisfy their employees. In particular, if I was in the position of a manager, I will begin by undertaking employee satisfaction survey in order to ascertain areas of dissatisfaction. Thereafter, I will implement the recommendations thereof in order to improve employee job satisfaction levels. Once implemented, I will undertake an employee satisfaction exit survey. The exit survey will help in evaluating the success of the strategies employed with the aim of boosting employee job satisfaction

Cohesive Groups Essay Example for Free

Cohesive Groups Essay In general terms, a group is said to be in a state of cohesion when its members possess bonds linking them to one another and to the group as a whole. Groups that possess strong unifying forces typically stick together over time whereas groups that lack such bonds between members usually disintegrate. Advantages of cohesive groups Firstly, members of cohesive groups tend to communicate with one another in a more positive fashion than non cohesive groups. As a result, members of cohesive groups often report higher levels of satisfaction and lower levels of anxiety and tension than members of non cohesive groups. Secondly, group cohesion has been linked to enhanced group performance in non laboratory based groups. This bi directional relationship is strongest when the members of a group are committed to the group’s tasks. Limitations of cohesive groups Membership in a cohesive group can also prove problematic for members. As cohesion increases, the internal dynamics (e.g., emotional and social processes) of the group intensify. As a result, people in cohesive groups are confronted with powerful pressures to conform to the group’s goals, norms, and decisions. In many instances these pressures to conform are so great that members suffer from groupthink. Individuals who refuse to yield to the ways of the majority are typically met with additional negative consequences, including hostility, exclusion, and scape goating. Furthermore, group cohesion can trigger distress and mal adaptive behavior in members following changes to the structure of the group (e.g., loss of a member). The five stage model of group development Stage 1: Forming In the Forming stage, personal relations are characterized by dependence. Group members rely on safe, patterned behavior and look to the group leader for guidance and direction. Group members have a desire for acceptance by the group and a need to be known that the group is safe. They set about gathering impressions and data about the similarities and differences among them and forming preferences for future sub grouping. Rules of behavior seem to be to keep things simple and to avoid controversy. Serious topics and feelings are avoided. The major task functions also concern orientation. Members attempt to become oriented to the tasks as well as to one another. To grow from this stage to the next, each member must relinquish the comfort of non threatening topics and risk the possibility of conflict. Stage 2: Storming The next stage, called Storming, is characterized by competition and conflict in the personal relations dimension an organization in the task functions dimension. As the group members attempt to organize for the task, conflict inevitably results in their personal relations. Individuals have to bend and mold their feelings, ideas, attitudes, and beliefs to suit the group organization. Because of fear of exposure or fear of failure, there will be an increased desire for structural clarification and commitment. Although conflicts may or may not surface as group issues, they do exist. Questions will arise about who is going to be responsible for what, what the rules are, what the reward system is, and what criteria for evaluation are. These reflect conflicts over leadership, structure, power, and authority. There may be wide swings in member’s behavior based on emerging issues of competition and hostilities. Because of the discomfort generated during this stage, some members may remain completely silent while others attempt to dominate. In order to progress to the next stage, group members must move from a testing and proving mentality to a problem solving mentality. The most important trait in helping groups to move on to the next stage seems to be the ability to listen. Stage 3: Norming In the norming stage, interpersonal relations are characterized by cohesion. Group members are engaged in active acknowledgment of all members’ contributions, community building and maintenance, and solving of group issues. Members are willing to change their preconceived ideas or opinions on the basis of facts presented by other members, and they actively ask questions of one another. Leadership is shared, and cliques dissolve. When members begin to know and identify with one another, the level of trust in their personal relations contributes to the development of group cohesion. It is during this stage of development that people begin to experience a sense of group belonging and a feeling of relief as a result of resolving interpersonal conflicts. The major task function of stage three is the data flow between group members: They share feelings and ideas, solicit and give feedback to one another, and explore actions related to the task. Creativity is high. If this stage of data flow and cohesion is attained by the group members, their interactions are characterized by openness and sharing of information on both a personal and task level. They feel good about being part of an effective group. The major drawback of the norming stage is that members may begin to fear the inevitable future breakup of the group; they may resist change of any sort. Stage 4: Performing The Performing stage is not reached by all groups. If group members are able to evolve to stage four, their capacity, range, and depth of personal relations expand to true interdependence. In this stage, people can work independently, in subgroups, or as a total unit with equal facility. Their roles and authorities dynamically adjust to the changing needs of the group and individuals. Stage four is marked by interdependence in personal relations and problem solving in the realm of task functions. By now, the group should be most productive. Individual members have become self assuring, and the need for group approval is past. Members are both highly task oriented and highly people oriented. There is unity: group identity is complete, group morale is high, and group loyalty is intense. The task function becomes genuine problem solving, leading toward optimal solutions and optimum group development. There is support for experimentation in solving problems and an emphasis on achievement. The overall goal is productivity through problem solving and work. Stage 5: Adjourning The final stage, adjourning, involves the termination of task behaviors and disengagement from relationships. A planned conclusion usually includes recognition for participation and achievement and an opportunity for members to say personal goodbyes. Concluding a group can create some apprehension in effect, a minor crisis. The termination of the group is a regressive movement from giving up control to giving up inclusion in the group.

Styles of Two Great American Writers Essay Example for Free

Styles of Two Great American Writers Essay A writer’s style distinguishes him from other writers. The style a writer uses to write a story clearly indicates the tone of a story,vital for the reader to understand the story. The style of a writer is made up of different traits and characteristics used to write the story. These traits and characteristics include and are not limited to symbolism, characterization, and other elements. When evaluating a literature piece for style one should analyze the following five elements: diction, images, details, language, and sentence structure. Two well-known American writers with completely different styles are Ernest Hemingway and William Faulkner. Hemingway and Faulkner’s similarities and differences in style become apparent when comparing and contrasting two of their famous short stories, â€Å"A Clean, Well-Lighted Place† by Ernest Hemingway and â€Å"Barn Burning† by William Faulkner. Diction involve word choices a writer makes for his story. These word choices may be used to achieve an overall feeling from a reader toward a story. Diction also reflects the writer’s attitude toward his subject. Ernest Hemingway’s choice of diction in â€Å"A Clean, Well-Lighted Place† are simple words that directly mean what they stand for. Hemingway uses simple, less complex words to describe both characters and setting. Examples of diction usage for setting are found in the title, â€Å"A Clean, Well-Lighted Place† (Hemingway 141) and â€Å"pleasant† (Hemingway 143). The two waiters describe the customer as a â€Å"clean old man a good client† (Hemingway 141). Hemingway’s choice of diction was blunt and to the point. The diction usage does not let the reader get funny ideas or leeway to think anything other than what Hemingway says. This element of Hemingway’s style reflects when the young waiter states the old man is â€Å"drunk† (Hemingway 141). The young waiter does not suggest he was tipsy or that he seemed intoxicated, he simply meant he was drunk. Hemingway uses the Spanish word for nothing, â€Å"nada† (Faulkner 145) to substitute words from the Lord’s prayer, in the old waiter’s speech of despair and loneliness to interrelate the theme. Building a sympathetic feeling from the reader toward the character’s way of seeing his lonely world as nothing, was Hemingway’s intention in doing so. In comparison to Ernest Hemingway, William Faulkner’s use of diction involves more complicated words due to the fact they’re slang and misspelled. Some misspelled words and slang are â€Å"kin hisn [and] hit† (Faulkner 145) which stand for the words can, his, and it. The reason for the use of a southern accent as diction is to set the mood of where the story takes place. Racist slurs such as â€Å"nigger† (Faulkner 146) also make up the diction of the story. Such racist slurs are used to build a certain attitude toward the character who says them. Faulkner describes Abner’s walk as â€Å"stiffly† (Faulkner 146) which also affects the reader’s feelings about a character. Sarty uses the word â€Å"bovine† (Faulkner 152) to describe his fat sisters. For many readers who are not familiar with the south or the last century the complex word usage in â€Å"Barn Burning† mean many things and nothing at times, this affects the reader’s attitude toward the story as a whole. The overall use of language in an entire story also makes up the writer’s style. Different types of language would be formal or informal, scientific or abstract, etc. There are no rules to correct language for a writer’s style. â€Å"A Clean, Well-Lighted Place† uses informal and conversational overall language. Incomplete statements and fragments that cast full scenes in form of conversation create informality of the story . â€Å"‘He hung himself with a rope. ’† (Hemingway 142) Also, a device Hemingway uses when a characters pauses and then speaks again in the following line make up the informal language. ‘â€Å"He’s drunk now,’ he said. ‘He’s drunk every night. †Ã¢â‚¬â„¢ (Hemingway 142) The language used in â€Å"Barn Burning† by William Faulkner includes colloquial, informal, with some interior monologue. The southern drawl creates a particular colloquial dialect. â€Å"‘Naw,’ he said. â€Å"Hit don’t hurt. Lemme be. ’† (Faulkner 147) Interior Monologue also makes up the element of language. â€Å"I could run on and on and never look back, never need to see his face again. † (Faulkner 155) is Sarty’s interior monologue. Faulkner uses interior monologue to prove Sarty was not allowed to express himself freely out of fear. The sentence structure used in a story has an impact on the reader’s attitude. The sentences may be structured in a variety of ways to get certain points and feelings across. Types of sentence structure include short, long, simple, complex, and even interrupted sentences. Sentence structure in Ernest Hemingway’s â€Å"A Clean, Well-Lighted Place† includes regular speech patterns. This would include short, choppy, simple and direct sentences. Examples of these types of sentences include, ‘â€Å"He has plenty of money. †Ã¢â‚¬â„¢ (Hemingway 141) and â€Å"‘Nothing. †Ã¢â‚¬â„¢ (Hemingway 141) Hemingway’s sentence structure also includes long, compound but simple sentences. â€Å"In the day the street was dusty, but at night the dew settled the dust and the old man liked to sit late because he was deaf and now at night it was quiet and he felt the difference. † (Faulkner 141) Formal, complex and long drawn-out sentences make up the sentence structure in â€Å"Barn Burning† by William Faulkner. Faulkner’s style, most of the time formal and excessively detailed, creates sentences extremely complicated that many times the best way to understand them is to go back and reread the sentence. â€Å"The boy, crouched on his nail keg at the back of the crowded room, knew he smelled cheese, and more: from where he sat he could see the ranked shelves close-packed with the solid, squat, dynamic shapes of tin cans whose labels his stomach read, not from the lettering which meant nothing to his mind but from the scarlet devils and the silver curve of fish-this, the cheese which he knew he smelled and the hermetic meat which his intestines believed he smelled coming in intermittent gusts momentary and brief between the other constant one, the smell and sense just a little of fear because mostly of despair and grief, the old fierce pull of blood. † (Faulkner 145) The five basic elements interrelate to create the style of a writer. After the reader understands style, the tone and the true meaning of a story become apparent. The style of both Ernest Hemingway and William Faulkner are different with only a few similarities. One style (Hemingway) being simple and to the point, the other (Faulkner) complicated and full of details. Although the differences are many, one apparent similarity is the informality in the overall language of both stories. Neither style ranks better than the other. The reader changes the value of each style depending on what they appreciate reading.

Discuss the challenges facing forensic scientists

Discuss the challenges facing forensic scientists GENETICS FOR IDENTIFICATION ESSAY Discuss the challenges facing forensic scientists for DNA-based identification of the remains of the victims of war or other conflicts (both civilian and military personnel). Illustrate with a range of examples. Discuss the challenges facing forensic scientists for DNA-based identification of the remains of the victims of war or other conflicts (both civilian and military personnel). Illustrate with a range of examples. Introduction The remains of victims of war often pose numerous challenges to forensic scientists enlisted to aid in the identification process using DNA analysis. Foremost, the remains of victims of war have frequently been buried for long periods of time, often over 50 years, and this can cause degradation and contamination of DNA. This affects the quality and quantity of extracted DNA, making it difficult to amplify and generate a genetic profile. This concise report highlights the problems facing forensic scientists during the analysis of war remains and the methods used to overcome some of these issues. DNA techniques The DNA techniques which can be used in DNA identification of skeletal bones include: mitochondrial DNA analysis (mtDNA); autosomal Short Tandem Repeat (STR) and Y-STR analysis; and mini-STR profiling. STRs, also known as microsatellites, are DNA repeat units between two and seven base pairs long that can easily be amplified by Polymerase Chain Reaction (PCR). The number of repeat units varies considerably amongst individuals, hence why sensitive STR analysis is highly discriminative for identification, even in degraded DNA samples (Butler et al., 2012). Fifteen years ago, results of a study where teeth were buried in soil for up to eighteen weeks revealed that mtDNA analysis using primers for the HV1 and HV2 regions generated the best results in comparison to other DNA techniques; thus mtDNA was a reliable method for the identification of skeletal remains (Pfeiffer et al., 1999). Developments in technology mean that analysis of mtDNA alone is now insufficient information for positive identification, and more specific methods are employed, such as STR analysis. Nevertheless, when nuclear DNA (nDNA) samples are too degraded to be processed using STR analysis, identification using mtDNA and hypervariable regions is used. This is because mtDNA is present in high copy numbers and the circular structure of mtDNA makes it more resistant to degradation, but again there are limitations (Higgins et al., 2013; Coble et al., 2005). Mitochondrial DNA is passed on maternally; therefore it will be the same throughout generations of females of the same maternal lineage and could identify a familial match to a sister or aunt, for example. However reference samples are solely restricted to maternal relatives, thus its discriminatory power is far less than an STR match (Lee et al., 2010). Finally, where female relatives are absent, Y-STR analysis can be carried out to identify paternal lineage of the Y-chromosome. Mutations within the Y-chromosome are possible, and they may occur between generations as well as within the same bone samples, particularly if they are old skeletal bones. This must be considered when making conclusions from Y-STR analysis as it may cause problems for the forensic scientist during the identification process (BoriĆ¡ et al., 2011). DNA samples from skeletal remains With war victim skeletal remains DNA sample options are minimal, but the preferential samples to obtain for DNA analysis are teeth and bones, which contain both nDNA and mtDNA. DNA in teeth is generally considered more protected against degradation and destruction than bones due their unique composition and their location in the jawbone protecting them against degrading exogenous organisms, making them useful for analysis decades after death. Additionally, they normally yield a higher quality of DNA than bones, and the results of a study by Pilli and colleagues looking into the effect of contamination on samples revealed that teeth have a greater refractory to contamination by exogenous DNA than bones (Higgins et al., 2013; Pilli et al., 2013 respectively). Another reason for utilising teeth is because there are a number of sources of DNA within the tooth, including dentine, and this dentine powder is rich in mtDNA (Muruganandhan et al., 2011). Nevertheless, both of these samples are used because the internal content is unlikely to be contaminated with contemporary DNA compared to other samples, and the recovery of one or both of these is observed in a number of case studies identifying war victims (Lee et al., 2010; MarjanoviĆ¡ et al., 2007; AndelinoviĆ¡ et al., 2005; Ivanov et al., 1999). However, although these sources of DNA are the most likely to be preserved over time, DNA analysis can still be affected by the presence of PCR inhibitors (for example environmental and biological chemicals), insufficient quantity of DNA material, and high levels of DNA degradation (MarjanoviĆ¡ et al., 2007). DNA degradation DNA degradation and PCR inhibitors occasionally cause allele and/or locus dropout, thus not reflecting the true profile of the individual and can cause problems for forensic scientists during the analysis of genotypic DNA profiles, especially if heterozygotes are interpreted as homozygotes (Coble et al., 2005). Exposure to environmental conditions affects DNA, and factors such as heat and humidity affect the rate of DNA degradation and the resulting quantity of DNA, with cooler temperatures delaying the degradation process. This DNA degradation caused by endogenous intracellular enzymes results in smaller DNA fragments sizes and may also cause base mutations (Higgins et al., 2013). Despite the development of sensitive DNA typing identification methods, in some cases excessive DNA degradation can still pose an issue, as seen in Lee et al., 2010. One sample (SR0014) had an exceptionally low DNA yield for both extractions, 28.7 ±20.69 pg/ µL and 27.2 ±16.81 pg/ µL, in comparison to the average yield of 217.5 pg/ µL and 199.1 pg/ µL, respectively (table 1). This DNA yield produced genotypic results at eleven autosomal STR loci; however nine of these were homozygotic and this was interpreted as allelic dropout, which in turn causes problems for profile determination and does not allow for identity inclusion or exclusion. Another example of degraded DNA amplification being below 100% was seen using AmpFISTR Yfiler amplification, which revealed only 34 out of 49 profiles, and MiniFiler which produced 40 out of 49 genetic profiles, with the other profiles being incomplete, likely due to only small amounts of degraded DNA being amplified (BoriĆ¡ et al., 2011). Table 1 – DNA concentrations (pg/ µL) extracted from twenty-one skeletal samples belonging to Korean War victims. Two DNA samples were extracted from each bone sample and quantified to try and replicate the profile for consistency and to highlight any contamination issues. (See Lee et al., 2010. Available at: http://onlinelibrary.wiley.com/doi/10.1111/j.1556-4029.2010.01411.x/pdf. Last accessed on: 24th February 2014) DNA extraction Another issue facing forensic scientists is the quantity of DNA extracted from bones or teeth. Degraded samples offer a paucity of template DNA concentration, hence the smaller PCR products (Ivanov et al., 1999). If precise procedures are not employed then DNA essential for producing a genotypic profile may be lost, and methods are required to maximise the potential from the extraction process. Recently, developments in such methods have massively impacted on the success of obtaining profiles from skeletal remains which are highly degraded. Extraction techniques employed by forensic scientists to overcome this challenge include the standard organic (phenol/chloroform) method, the PrepFiler Forensic DNA Extraction kit, the Qiagen DNA extraction procedure, and a large-scale silica based extraction method, with a minimum of two independent extractions for each sample usually taken. Lee and colleagues used the latter in 2010 alongside demineralisation to maximise the DNA yield, and the p ositive effect of this method was reflected in the high DNA yields observed following quantification, with all samples, except two, being greater than 50pg/ Ã‚ ­L (Lee et al., 2010). Additionally, during the extraction of DNA from 109 bone samples from victims of war in mass graves in Croatia, an advanced extraction method, alongside the phenol/chloroform method, was used. The standard method yielded 20-100ng of extracted DNA across samples, whilst the advanced Promega DNA IQ system produced 20-200ng of DNA (AndelinoviĆ¡ et al., 2005). Contamination The sensitivity of new DNA typing methodologies to minute amounts of DNA brings with it the challenge of contamination. Remains buried for a long period of time and then excavated for analysis are subject to natural cross contamination from foreign material from the surrounding environment as well as from human handling, and this can affect the validity of the findings and interfere with DNA profiles. Thus, procedures are implemented to remove any foreign matter from the outer surface which may contaminate the probe extracting the DNA from the core of the bone or tooth. Common protocols forensic scientists use to overcome the issue of contamination on bones include sanding down the outside of the bone, washing it in mild detergent, irradiating it with UV light and storing it at -20oC until it is required for DNA extraction (Ossowski et al., 2013; BoriĆ¡ et al., 2011; Lee et al., 2010; MarjanoviĆ¡ et al., 2007; Imaizumi et al., 2002). However, in cases where excess contamination has occurred from archaeological handling and repeated excavation and reburial, it is impractical for forensic scientists to remove this extent of contamination, further limiting the samples which can be used for DNA. It is of paramount importance that sterile environments are used for sample analysis and that gloves are worn when handling bones to avoid contamination from humans, and gloves should be changed between the handling of bones belonging to different individuals to avoid DNA transfer. Another issue regarding contamination is the processing of ancient skeletal samples in proximity to contemporary relative reference samples, therefore, DNA from these two sample types should be extracted and amplified in separate laboratories. The majority of studies referenced in this report have not stated whether they adhered to this, with the exception being Ossowski et al., 2013 who reported that all laboratory staff wore masks, lab overalls and sterile gloves and everyone who handled the samples had previously had their DNA sample taken for reference purposes. As a result of the strict procedures implemented, no contamination was seen throughout the examination process, and they successfully identifi ed two individuals through DNA analysis when it was determined that they could not be identified through anthropological methods (Ossowski et al., 2013). Finally, a complete record of everyone that has handled the samples pre- and post-excavation should be kept (Pilli et al., 2013). Issues with mass grave DNA samples A further problem forensic scientists are confronted with is the high number and poor quality of the remains discovered, particularly in mass graves, due to wars being open events with large numbers of unknown individuals involved. The violent nature of wars and conflicts and circumstances of death often renders a number of remains fragmented, meaning that prior to DNA analysis, an anthropologist must examine and match bones suspected to belong to the same individual. This should be considered during DNA analysis in case different profiles arise from bones supposedly belonging to the same individual. Furthermore, explosives can cause bones to become incinerated, damaged and carbonised which will affect DNA extraction and amplification (Ivanov et al., 1999). This issue was presented during the recovery of approximately 10,000 skeletal remains, belonging to approximately 53 war victims in 2009, more than sixty years after World War II. In the majority of cases, it was impossible to conclude which bones belonged to one individual due to the vast number of separated, damaged and intermingled bones and the small grave area to which they were confined. Additionally, a number of victims showed obvious gunshot wounds, which further shattered the skull bones into multiple fragments (BoriĆ¡ et al., 2011). DNA reference samples Prior to DNA analysis, it is paramount that circumstantial investigative research is carried out to narrow down and establish possible victims in the grave to allow for identification of their surviving relatives. This is problematic in the first instance if ante-mortem records of soldiers have not been kept. It is reasonable to assume that relatives of military personnel would be easier to identify, compared to civilians, because records are often kept of the soldiers that fought in a war, as well as when they were reported missing or pronounced dead. Reference swabs are required from these presumptive relatives to compare their DNA profiles with the profiles obtained from the unknown remains to determine if a familial match is seen. Ivanov et al., 1999 reported absent DNA samples on record during their involvement in the identification of the remains recovered following the Chechen War (1994-1996). Few remains were available due to the lethal force of missiles, and this was an additional problem to the lack of DNA samples on record, which meant the absence of comparative reference samples. Accordingly, the timely process of locating potential relatives and collecting their DNA samples began. In comparison to some cases where excavated war remains are over 60 years old (MarjanoviĆ¡ et al., 2007), these remains were excavated three years after the end of the war, however skeletonisation and advanced decomposition, with mummification, was still observed. Bones had been scavenged by animals, further exposing them to contamination alongside the reported careless excavation of the bodies (Ivanov et al., 1999). Mini-STRs Applying mini-STR loci to severely degraded DNA samples is effective in genotyping nDNA profiles that would otherwise yield a negative result with standard STR kits, which use STR loci of up to 250 base pairs and are likely to cause loss of signal (AndelinoviĆ¡ et al., 2005). In comparison, mini-STR technology can amplify loci with alleles that have fewer than 150 base pairs and works by annealing primers as close as possible to the STR repeat region, creating the smallest possible amplicon (Figure 1) (Martin et al 2006; Pizzamiglio et al., 2006). When there is not enough intact DNA to produce full profiles using the larger CODIS loci, mini-STR loci markers are small enough to amplify alleles less than 150 bp in length (Hill et al., 2008). It is important to remember that mini-STRs are designed to profile high quantity, low quality, degraded DNA and should not be used for small amounts of DNA. Fig. 1 – Mini-STR analysis uses primers which anneal as close as possible to the STR repeat region along the genome, creating a small amplicon to be amplified. Successful application of mini-STRs was seen in 2010 when skeletal remains from the Korean War were subject to DNA analysis in the hope to identify the 55 year old remains of the missing casualties (Lee et al., 2010). Twenty-one skeletal samples were extracted from the remains of the victims of the Korean War and, following decontamination, were subjected to PCR amplification and sequencing of the mtDNA HV region, and PCR amplification of autosomal STRs and Y-STRs using common STR kits and in-house miniplex plus systems that use smaller amplicons to optimise the genetic material from degraded samples. Results revealed that mtDNA hypervariable regions were efficiently amplified and determined in all 21 samples. A combination of AmpFISTR Identifiler alongside size-reduced amplicons in AmpFISTR MiniFiler and the in-house miniplex NC01 plus system for autosomal STR, successfully genotyped 17.2 loci out of 18, and the miniplex NC01 system showed 100% success in genotyping the four loci du e to the reduced amplicon size. Additionally, twenty samples were successfully genotyped at 11 or more loci using standard STR kits, but with the in-house system, they were genotyped at 15 or more loci. The results of amplifying autosomal STRs showed the importance of mini-STRs when working with highly degraded DNA, as reduced-sized amplicons genotyped samples with a low quality of DNA in comparison to the standard kits. The same success of genotyping samples of poor quality DNA was seen using the Y-miniplex plus system in conjunction with AmpFISTR Yfiler during Y-STR genotyping (Lee et al., 2010). Profiles were also generated using mtDNA PCR amplification and miniplex NC01 analysis using the buccal samples provided by the 24 suspected relatives (Lee et al., 2010). Smaller quantities of template DNA were extracted from the relatives, as although more DNA was available, it was of a higher quality compared to the degraded unknown samples, so a smaller amount was required. If too much high quality template DNA is used, excess peaks and spurious bands would be observed, making the results unclear (Ivanov et al., 1999), and alternatively too little, or degraded DNA, may reduce the height of some alleles so the peak heights may be too low to distinguish from background noise (NFSTC Science Serving Justice, 2007). Conclusion In conclusion, it is evident that DNA degradation affecting the quality and quantity of DNA, fragmentation of bones, numbers of bones in mass graves, contamination and poor extraction procedures are all challenges that forensic scientists face during DNA based identification of skeletal war victim remains. However, as efficient DNA extraction methods to optimise the template DNA concentration are advancing, and technology is consistently being refined to develop methods such as the mini-STR system, identification of war victims using DNA analysis, alongside anthropological measures, is becoming more successful. Word count: 2668 Page | 1

Barbie Essay -- essays papers

Barbie Since the beginning of time, toys have often been an indicator of the way a society behaves, and how they interact with their children. For example, in ancient Greece, artifacts recovered there testify that children were simply not given toys to play with as in the modern world. The cruel ritual of leaving a sick child on a hillside for dead, seems to indicate a lack of attention to the young (Lord 16). The same is true of today’s society. As you can see with the number of toy stores in our society, we find toys of great value to our lives and enjoy giving them to children as gifts. Ask just about any young girl what she wants for Christmas and you’ll undoubtedly get the same answer: â€Å"A Barbie.† But what exactly has caused this baby boomer Barbie craze, and how did the entire world get so caught up in it? The answer lies in Ruth Handler’s vision for the first children’s adult doll. Mrs. Handler’s eleven and one-half-inch chunk of plastic began causing problems even before it’s public debut in 1959, yet has managed to become one of America’s favorite dolls. Ruth Handler and her two young children, Barbara and Ken, were merely sightseeing in Lucerne, Switzerland, when Mrs. Handler first saw the doll she herself had been trying to create (Lord 29). In the window of a small gift shop was an eleven and one-half-inch tall plastic doll with a slender woman’s body and a long blond ponytail. Her name was Lilli (â€Å"Bad Girl† 1). She had been created from a cartoon character in a West German tabloid similar to the National Inquirer (Lord 8). Dressed provocatively, and with a seductive look in her eye, Lilli had become a â€Å"popular pornographic gag gift for men† (â€Å"Bad Girl† 1). Excited to see her long-time idea a reality, Mrs. Handler bought three of the dolls and hurried home to begin work on her own doll (â€Å"Bad Girl† 2). It was 1956, and within three years, Mattel Creations began marketing the â€Å"teenage fashion model† as â€Å"a new kind of doll from real life† (Tosa 30). The new doll, deemed â€Å"Barbie†, was named after her own daughter Barbara, who’s many years of play with paper dolls had actually inspired her to begin designing the three-dimensional adult doll (Lord 30). Though Mrs. Handler’s version of the doll was not as racy or alluring as Lilli, her imitation of the â€Å"German streetwalker† would come back to haunt her many years later (†Bad ... ...body and a new focus, she has plans to become even more spectacular with many new adventures and many new friends. Barbie’s new look includes a wider waist, smaller hips, a less â€Å"torpedo-like† bust, and flat rather than pointy-toed feet (â€Å"Bad Girl† 3). She is also playing an active role in new research in prosthetics. Jane Bahor, a woman who makes replacement body parts, had experimented with the plastic knee joints in Barbie’s legs. She has found that they work well as prosthetic fingers for her patients because they â€Å"are more realistic-looking and useful†. So far, Bahor has provided the replacement joints for more than a dozen of her patients and has been extremely successful in her studies (â€Å"Bad Girl† 27). As Barbie gets ready to turn the big 4-2, it is unreal to think that she has completed her last makeover. No doubt that as time changes and people’s attitudes towards life change, this timely doll will also be forced to adjust to the needs of society. With more than 75 successful careers, her own official website, and a namesake magazine, this little doll has become more than a child’s plaything. Whether we love her or hate her, she will always be a part of us all.

Essay --

The War of 1812, which is also called the second war for independence is really important because this is where our freedom, liberty and democracy was earned. In this war, the great Britain and America tried to protect their land and fought for their country. Compared to the other wars like World War II, not many people died, but it certainly brought some changes and even the beginning of the new America. How did the war start? In 1803, Britain was involved in some situations with France. At this time, the Emperor of France was Napoleon Bonaparte. Later on, Britain and France was in a war. American people traded their goods with France but Britain stopped it. This became a problem for Americans because they had to stop in Britain first and do whatever the British generals demanded. The British treated Americans as if they were still under their control. After few months, they created new treaties, in which Americans could not trade with Europe, unless they were English ships. Other causes of the war were the impressments of the American sailors and the British’s help for the Indians. Every year, British lost thousands of sailors. They thought that if they will lose the battles at sea, then they would definitely lose the war. Then, they started to impress American sailors to be in the British Royal navy. If any of the American sailors refused to do what the British said, then British army attacked their ships. Third reason was that the Indian warfare on the frontier. Indian people alliance's from Alabama to Canada with the British’s help. They gave the Indian people the supplies to fight against americans, so that America could not expand. In 1810, two war hawks named John C Calhoun and Henry Clay stood up for the wa... ...ough, women got paid less than men, they still received some status. Shipping goods was a huge problem before and during the war, but it was solved by the improvements in the transportation system. This system helped the goods to reach faster at it’s destination. There were more railroads, steam ships, which carried goods to other states, turnpikes, and canals which allowed everything to work at a much faster pace than it used to. Overall, the war of 1812 brought us freedom and liberty. The nation, for which all of the soldiers fought, was recognized as a great, free and independent land. This war helped Americans to realize that they can protect their country if they get threatened by foreign countries. Americans started to settle their western country. Shipbuilding and commerce flourished. The United States began to rise to the top position of a world’s power.

The Dirty South :: essays research papers

The Dirty South During the times of the Civil Rights Movement the black communities of Birmingham, Alabama suffered severely due to the notorious acts of racism geared towards them simply because they were black. They boldly endured beatings, lynching, bombings, and demeaning treatment from the white community and especially from the Clan. The September 15, 1963 racially motivated bombing of the Birmingham’s Sixteenth Street Baptist Church, which resulted in the deaths of four innocent black girls, was one of the darkest moments of the Civil Rights Movement and perhaps one of the darkest days in Birmingham, Alabama’s history. Betty Blackman was born and raised in Birmingham. Her life was engulfed by the racism and left her with dramatically scaring memories of Birmingham.   Ã‚  Ã‚  Ã‚  Ã‚  I grew up in the city of Birmingham, Alabama. I was born there December 11, 1947. Most of the memories I have of growing up there are the most painful memories that I have, which is strong racism and living in every day fright. I remember not being able to drink out of the cleaner water fountains around town; they were for the white people. The water fountains that were available to us were few, far between and very filthy. The black people were treated like dogs in Birmingham. I remember having to enter of the all stores and restaurants in town through the back entrances. One place I remember so vividly was a restaurant called Stadium Grill. We ordered food there every week while we were doing the wash across the street at the Laundromat. We enter in the back door into a very tiny poorly lit room. There were no tables or chairs for us to sit and eat there, it wasn’t allowed. There was only a small window to which we placed our orders and left. The front of the restaurant was large; it had tables with real cloth coverings and beautiful flowers sitting in the middle of the tables. I never once stepped foot inside the front of that restaurant. The way our communities were much different than they are now. Black people were not allowed to live among the white people. The white people lived in big lavish homes on the far east side of town and the blacks lived on the west side of town in small run down homes. There was, however, one subdivision that the wealthier black families lived. The name of it was the Goldwire Area and even now it never compares to the homes in which the poverty stricken families live now. The Dirty South :: essays research papers The Dirty South During the times of the Civil Rights Movement the black communities of Birmingham, Alabama suffered severely due to the notorious acts of racism geared towards them simply because they were black. They boldly endured beatings, lynching, bombings, and demeaning treatment from the white community and especially from the Clan. The September 15, 1963 racially motivated bombing of the Birmingham’s Sixteenth Street Baptist Church, which resulted in the deaths of four innocent black girls, was one of the darkest moments of the Civil Rights Movement and perhaps one of the darkest days in Birmingham, Alabama’s history. Betty Blackman was born and raised in Birmingham. Her life was engulfed by the racism and left her with dramatically scaring memories of Birmingham.   Ã‚  Ã‚  Ã‚  Ã‚  I grew up in the city of Birmingham, Alabama. I was born there December 11, 1947. Most of the memories I have of growing up there are the most painful memories that I have, which is strong racism and living in every day fright. I remember not being able to drink out of the cleaner water fountains around town; they were for the white people. The water fountains that were available to us were few, far between and very filthy. The black people were treated like dogs in Birmingham. I remember having to enter of the all stores and restaurants in town through the back entrances. One place I remember so vividly was a restaurant called Stadium Grill. We ordered food there every week while we were doing the wash across the street at the Laundromat. We enter in the back door into a very tiny poorly lit room. There were no tables or chairs for us to sit and eat there, it wasn’t allowed. There was only a small window to which we placed our orders and left. The front of the restaurant was large; it had tables with real cloth coverings and beautiful flowers sitting in the middle of the tables. I never once stepped foot inside the front of that restaurant. The way our communities were much different than they are now. Black people were not allowed to live among the white people. The white people lived in big lavish homes on the far east side of town and the blacks lived on the west side of town in small run down homes. There was, however, one subdivision that the wealthier black families lived. The name of it was the Goldwire Area and even now it never compares to the homes in which the poverty stricken families live now.